Table3: Changes in total
free amino acids, total protein, glycogen level, and nucleic acid level (DNA
and RNA) in nervous and hepatopancreas tissues of freshwater snail Lymnaea acuminata after exposure to
sub-lethal doses (40% and 80% of LC50 of 24h) of methanolic extract
of stem bark of Lantana indica after
96h exposure periods in pond.
|
Parameter
|
Tissue
|
Control
|
40% of LC50
|
80% of LC50
|
7 days of the withdrawal
|
|
Amino acid
|
Nervous
|
17.0± 0.01
(100)
|
20.0±0.01*
(118)
|
23.0±0.01*
(135)
|
18.6±0.03**
(109)
|
|
Hepatopancreas
|
18.2±0.01
(100)
|
22.6±0.01
(124)
|
25.1±0.01*
(138)
|
19.5±0.02**
(107)
|
|
Protein
|
Nervous
|
21.0± 0.01
(100)
|
16.4±0.01*
(78)
|
12.4±0.01*
(59)
|
19.7±0.02**
(94)
|
|
Hepatopancreas
|
25.0±0.01 (100)
|
19.0±0.01*
(76)
|
14.0±0.01*
(56)
|
23.9±0.01**
(96)
|
|
Glycogen
|
Nervous
|
1.4± 0.01
(100)
|
1.0±0.01*
(71)
|
0.7±0.01*
(50)
|
1.3±0.01**
(93)
|
|
Hepatopancreas
|
1.1±0.01 (100)
|
0.77±0.01*
(64)
|
0.52±0.01*
(47)
|
1.0±0.01**
(91)
|
|
DNA
|
Nervous
|
12.4±0.01
(100)
|
10.3±0.01*
(83)
|
8.2±0.01*
(66)
|
11.5±0.01**
(93)
|
|
Hepatopancreas
|
13.0±0.01
(100)
|
9.9±0.01*
(76)
|
8.5±0.01*
(65)
|
12.4±0.01**
(95)
|
|
RNA
|
Nervous
|
19.3±0.01
(100)
|
14.4±0.01*
(75)
|
12.1±0.01*
(63)
|
18.6±0.02**
(96)
|
|
Hepatopancreas
|
17.8±0.01
(100)
|
13.0±0.01*
(73)
|
10.7±0.01*
(60)
|
16.9±0.01**
(95)
|
|
Protease
|
Nervous
|
0.32±0.02
(100)
|
0.41±0.01* (128)
|
0.45±0.02* (141)
|
0.34±0.01**
(106)
|
|
Hepatopancreas
|
0.46±0.01
(100)
|
0.55±0.01*
(120)
|
0.63±0.03*
(137)
|
0.50±0.01**
(109)
|
·
All these values are ± SE of six
replicates.
·
Values in parenthesis are percent
change with control taken as 100%.
·
Data were analysed through
student’s‘t’ test.
·
*, shows significant (P<0.05)
changes, when treated groups were compared with controls.
·
**, shows significant (P<0.05).
Student ‘t’ test was applied between 80% of 24h LC50 and withdrawal.
·
Levels of total free amino acids,
total protein, nucleic acid (DNA and RNA) are expressed in µg/mg and glycogen
level are expressed in mg/g and activity of protease in micromoles of tyrosine equivalents/mg protein /h.
